Development of multiplex PCR assay for species-specific detection and identification of Saprolegnia parasitica
Saprolegnia parasitica is the most important pathogen under the genus, Saprolegnia which causes devastating oomycete diseases in freshwater fish.At present, the most common molecular method for identification of Saprolegnia species is sequencing of ribosomal DNA internal transcribed spacer (rDNA-ITS) region.In this study, a highly sensitive multipl